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You are here: Home / Articles / Infiltrating the Disc: Mast Cells & Back Pain

Infiltrating the Disc: Mast Cells & Back Pain

November 6, 2017 • By Lara C. Pullen, PhD

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Mast cells play an important role in the pathogenesis of degenerative musculoskeletal diseases and chronic pain conditions. Recent work has revealed that mast cells perform a critical function in the pathophysiology of osteoarthritis. Now, scientists have turned their attention to the role of mast cells in low back pain.

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Researchers looked specifically at the intervertebral disc, which contains three integrated structures: the inner gelatinous nucleus pulposus (NP), the outer lamellar annulus fibrosus (AF) and the cartilaginous endplates (EP) that connect the invertebral discs to the vertebral bodies. A healthy intervertebral disc is considered immune privileged, and the disc—in particular the AF—acts as a physical and biochemical barrier to inflammation and immune cell infiltration. However, during aging, degeneration and/or injury, mast cells can enter the intervertebral disc. Although scientists have documented the presence of mast cells in painful discs, the role of mast cells in low back pain and intervertebral disc degeneration is still not well understood.

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Recent research published online on Oct. 2 in Scientific Reports revealed that mast cells can infiltrate intervertebral disc cells and play a key role in their degeneration.1 The investigators designed their study to better understand the presence and role of mast cells within the intervertebral disc. Matthew G. Wiet, a medical student at Ohio State University, Columbus, and colleagues found that not only can mast cells affect the function of intervertebral disc cells, but intervertebral disc cells can also affect the function of mast cells.

“To our knowledge, this is the first study to explicitly explore the presence and function of mast cells in the intervertebral disc,” write the authors in the conclusion of their paper. “Here we demonstrated that mast cells are present in the painful [intervertebral disc] and that they likely play a key role in degeneration of the [disc]. We provide evidence that mast cells are able to 1) stimulate an inflammatory and catabolic phenotype in [intervertebral disc] cells specifically NP and EP cells, and vice versa, and 2) that healthy annulus fibrosus cells can inhibit activation of mast cells and a pro-angiogenic phenotype in these cells.”

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The investigators used immunohistochemistry and 3D in vitro cell culture methods to characterize mast cell–intervertebral disc cell interactions. They began their work by using immunohistochemistry staining to investigate the presence of mast cell-specific markers within the human intervertebral disc. They examined painful intervertebral discs that had been surgically removed from patients and compared them with cadaveric intervertebral discs. Study limitation: The researchers assumed the cadaveric discs came from individuals who had died without back pain because the discs were intact upon the patient’s death. The researchers did not indicate that any efforts were made to determine whether the individuals had actually been living with pain prior to their deaths. This flaw appears to be reflected in the data: 19.3% of cadaveric samples had mast cells.

The researchers found painful discs were more likely (53.3%) to have mast cells than were cadaveric autopsy samples (19.3%). In other words, discs that were associated with lower back pain were more likely to have mast cells than cadaveric discs. When the investigators looked more closely at the structures of the intervertebral discs, they found the NP of the intervertebral discs removed from patients suffering lower back pain tended to have increased expression of stem cell factor relative to cadaveric control NPs. The authors concluded from this that the mast cells in the painful discs had been upregulated.

Next, the researchers evaluated the effect of soluble factors secreted from mast cells on bovine intervertebral disc cells and found the presence of mast cells was associated with significantly increased expression of IL-6 and the enzyme ADAMTS5 in NP cells relative to bovine intervertebral disc cells cultured in the absence of mast cells. They then asked the reverse question: “Could the intervertebral discs secrete factors that could influence the mast cells?” They found that mast cells placed on healthy bovine annulus fibrosus cells had decreased expression of key inflammatory and pro-angiogenic genes. The mast cells also demonstrated less neo-angiogenesis formation when they were placed on healthy bovine annulus fibrosus cells.

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The authors conclude their paper by suggesting that mast cells may be a potential target for low back pain therapy.


Lara C. Pullen, PhD, is a medical writer based in the Chicago area.

Reference

  1. Wiet MG, Piscioneri A, Khan SN, et al. Mast cell-intervertebral disc cell interactions regulate inflammation, catabolism and angiogenesis in discogenic back pain. Sci Rep. 2017 Oct 2;7(1):12492. doi: 10.1038/s41598-017-12666-z.

Pages: 1 2 | Multi-Page

Filed Under: Conditions, Osteoarthritis Tagged With: Back pain, intervertebral disc, low back pain, mast cell, OsteoarthritisIssue: December 2017

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